(ININ)- Iranian researchers created a new generation of Insulin loaded nanoparticles and studied its effect on Caco-2 cell monolayers of intestine.
The team of researchers introduced a progressed
method to protect peptide drugs from rapid hydrophilic and enzymatic degradation
as such drugs are in contact with the gastrointestinal fluids.
"The main purpose of the research project is to protect insulin absorption in case of oral intake, and study its effect on intestine's cancer cells," Dr. Asal M. M. Sadeghi, a member of the research team said.
She added that the method used in creating insulin loaded nanoparticles, called as Polyelectrolyte Complexation, causes no harm to proteins.
In an appropriate pH, negatively charged insulin reacts directly with positively charged Chitosan polymer, leading to formation of nanoparticles.
The characteristics of insulin nanoparticles including the particles' size, thire Zeta potential, the amount of insulin release from the nanoparticles (in different pH values), their transepithelial electrical resistance (TEER) and their Permeability have been studied during the research.
The project was carried out under the guidance of Prof. H. E. Junginger (Leiden University, The Netherlands), Dr. M. Rafiee Tehrani (Tehran University of Medical Sciences) and Dr. M. R. Avadi (Hakim Pharmaceutical Company, Tehran).
Details of the research project were recently published in the European Journal of Pharmaceutics and Biopharmaceutics as well as the International Journal of Pharmaceutics.
... Payvand News - 02/12/09 ... --